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Ghosh, S. K.
- DNA Barcode of Royal Bengal Tiger (Panthera tigris) and Domestic Cat (Felis Sylvestris Catus) Using Own Designed PCR Primers
Abstract Views :654 |
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Authors
Affiliations
1 Department of Biotechnology, Assam University, Silchar-788011, IN
2 College of Veterinary Science, Khanapara, Guwahati, Assam, IN
3 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, IN
1 Department of Biotechnology, Assam University, Silchar-788011, IN
2 College of Veterinary Science, Khanapara, Guwahati, Assam, IN
3 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, IN
Source
Journal of Environment and Sociobiology, Vol 10, No 1 (2013), Pagination: 19-24Abstract
The DNA barcoding of Felidae (cat family, using mitochondrial Cytochrome oxidase subunit-I (COI) region opens a new vista for molecular identification of different life forms on our planet and caters a boon for cataloguing of taxa. In the present study, an attempt is made to develop a set of universal primer (forward COI-Fl and reverse COI-R1) for PCR amplification of COI gene for higher mammals and validate by successful amplification and subsequent sequencing of the gene in Royal Bengal Tiger (Panthera tigris) and domestic cat (Felts sylvestris catus). The sequenced data are submitted in GenBank (Accession No FJ185309 and FJ185310) as DNA barcode of the species. The designed primers opened a new hope in mitigating a long standing problem of species identification due to poaching of wild animals, adulteration of meat and animal products and purity testing as it can amplify wide range of barcode sequences of higher mammals. The obtained sequences along with other relevant sequences already existed in global database are analyzed to determine phylogenetic relationship and discussed.Keywords
DNA Barcoding, Royal Bengal Tiger, COI Gene, PCR, NCBI.- Revealing Genetic Diversity of Clarias batrachus Using DNA Barcode
Abstract Views :244 |
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Authors
Affiliations
1 DNA barcode and Genomics Laboratory, Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
1 DNA barcode and Genomics Laboratory, Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
Source
Journal of Environment and Sociobiology, Vol 10, No 1 (2013), Pagination: 25-32Abstract
Clarias batrachus is a species of freshwater catfish widely used for human consumption. Increasing demand of this species worldwide, coupled with its ability to survive in wide range of environmental conditions, has led to its introduction in many countries. This widespread translocation and distribution of C. batrachus have resulted in the rise of wide variety of haplotypes of this species. Nevertheless, there has been constant decrement in their population density in the last two decades. Thus, to endeavour conservation of the species we employ molecular technique of DNA barcoding in solving the standing problem of crypticism and haplotype sharing of the species. A better knowhow of the genetic makeup of the unique identifier region, that is, the 648 basepair region of COI DNA barcode will help to differentiate among closely related species and identify endemic species. In this study, a comparative analysis of C. batrachus from different regions in India and other parts of world shows presence of distinct haplotypes in different geographical locations. We also present a descriptive study of the various species of Clarias genus that have been barcoded in India till date. Our results also solve the dilemma of considering some species as synonymy of C. batrachus.Keywords
Clarias batrachus, Cytochrome C Oxidase Subunit I (COI), Genetic Diversity, Kimura's Two Parameter (K2P), Mean Divergence, Neighbour Joining Tree.- DNA Passport of Indian Catmint (Anisomeles indica) from Northeast India
Abstract Views :298 |
PDF Views:1
Authors
Affiliations
1 DNA barcode and Genomics Laboratory, Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, IN
1 DNA barcode and Genomics Laboratory, Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, IN
Source
Journal of Environment and Sociobiology, Vol 10, No 1 (2013), Pagination: 33-36Abstract
The plant Anisomeles indica (Lamiaceae) is commonly known as 'Indian Catmint' which is traditionally used as an anticancer and anti-inflammatory and as a source of new anti-metastatic agent for food and pharmaceutical industries. Here, we adopt the plant DNA barcoding technique to develop species level DNA passport for Anisomeles indica based on matK sequence (-800bp) of chloroplast DNA isolated from Anisomeles indica with subsequent amplification and sequencing for species level identification. Our result demonstrated 100% similarity with other Anisomeles indica sequences retrieved from global databases. The phylogeny analysis revealed that Anisomeles indica formed distinctive clusters with its different closely related species assigning correct taxonomic position. We propose that matK gene may be used as DNA passport for medicinal plants.Keywords
Anisomeles indica, DNA Passport, MatK, Sequence Homology, Species Level.- MATK Sequence Based Plant DNA Barcoding Failed to Identify Bambusa (Family:Poaceae) Species from Northeast India
Abstract Views :447 |
PDF Views:1
Authors
Affiliations
1 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 Tripura University, Agartala, Tripura, IN
1 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 Tripura University, Agartala, Tripura, IN